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Función de la GTPasa Npa3 en la levadura Saccharomyces cerevisiae

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dc.contributor Roberto Sánchez Olea;0000-0002-6523-7821 es_MX
dc.contributor.advisor Sáncez Olea, Roberto
dc.contributor.advisor Calera Medina, Mónica Raquel
dc.contributor.advisor Riego Ruiz, Lina Raquel
dc.contributor.advisor Layseca Espinosa, Esther
dc.contributor.advisor Salgado Bustamante, Mariana
dc.contributor.author Mora García, Martín Antonio
dc.coverage.spatial México. San Luis Potosí. San Luis Potosí. es_MX
dc.creator Martín Antonio Mora García;781284 es_MX
dc.date.accessioned 2022-11-03T18:58:06Z
dc.date.available 2023-11-09
dc.date.available 2022-11-03T18:58:06Z
dc.date.issued 2022-11-09
dc.identifier.uri https://repositorioinstitucional.uaslp.mx/xmlui/handle/i/8053
dc.description.abstract The GPN-loop GTPase Npa3 is encoded by an essential gene in the yeast Saccharomyces cerevisiae. Npa3 plays a critical role in the assembly and nuclear accumulation of RNA polymerase II (RNAPII), a function that may explain its essentiality. Genetic interactions describe the extent to which a mutation in a particular gene affects a specific phenotype when co-occurring with an alteration in a second gene. Discovering synthetic negative genetic interactions has long been used as a tool to delineate the functional relatedness between pairs of genes participating in common or compensatory biological pathways. Previously, our group showed that nuclear targeting and transcriptional activity of RNAPII were unaffected in cells expressing exclusively a C-terminal truncated mutant version of Npa3 (npa3ΔC) lacking the last 106-residues naturally absent from the single GPN protein in Archaea, but universally conserved in all Npa3 orthologs of eukaryotes. In order to gain insight into novel cellular functions for Npa3, we performed here a genome-wide Synthetic Genetic Array (SGA) study coupled to bulk fluorescence monitoring to identify negative genetic interactions of NPA3 by crossing an npa3ΔC strain with a 4,389-nonessential gene-deletion collection. This genetic screen revealed previously unknown synthetic negative interactions between NPA3 and 15 genes. Our results revealed that the Npa3 C-terminal tail extension regulates the participation of this essential GTPase in previously unknown biological processes related to mitochondrial homeostasis and ribosome biogenesis. es_MX
dc.description.statementofresponsibility Investigadores es_MX
dc.description.statementofresponsibility Estudiantes es_MX
dc.language Inglés es_MX
dc.publisher Facultad de Medicina es_MX
dc.rights Acceso Embargado es_MX
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0 es_MX
dc.subject GTPase Npa3 es_MX
dc.subject C-terminal deleted Npa3 es_MX
dc.subject Gpn1 es_MX
dc.subject synthetic genetic interactions es_MX
dc.subject synthetic lethal es_MX
dc.subject fluorescence es_MX
dc.subject mitochondria es_MX
dc.subject ribosome biogenesis es_MX
dc.subject.other BIOLOGÍA Y QUIMICA es_MX
dc.subject.other MEDICINA Y CIENCIAS DE LA SALUD es_MX
dc.title Función de la GTPasa Npa3 en la levadura Saccharomyces cerevisiae es_MX
dc.type Tesis de doctorado es_MX
dc.degree.name Doctorado en Ciencias Biomédicas Básicas es_MX
dc.degree.department Facultad de Medicina es_MX


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